corneal preservation medium

CORNEAL  PRESERVATION MEDIA

For short term storage (upto 96 hours at 4 degree   celsius)

MOIST CHAMBER

For maximum 1 day. Whole globe is kept in moist chamber. 

MODIFIED McCarey Kaufman (MK) MEDIUM :

Constituents:

*Tissue culture 199

*5% dextran

*N'-2 hydroxy ethyl piperazine Nethane sulfonic acid (HEPES) 15-20mmol/L.

*Penicillin G

*Gentamicin 200 microgms/ml

*Polymyxin B 50 micrograms/ml

*Phenol red as an indicator

*pH 7-7.5

*Osmolality 295-355 

For intermediate term storage (upto 15 days at 4 degree celcius)

LIKEROL

Constituents:

*Sodium pyruvate

*Glucose

*Vitamins

*Na, K, Cl and other electrolytes

* 2% chondritin sulfate

*HEPES

*Gentamicin, Penicillin, Streptomycin

*Phenol red as an indicator

OPTISOL GS

Constituents:

*TC199 + Minimum essential medium (base medium)

*Chondroitin sulfate 2.5%

*Dextran 40

*HEPES

*Gentamicin

*Streptomycin

*Cholesterol

*ATP

*Iron

*Sodium bicarbonate

*Sodium pyruvate

*Antioxidants

*Non easential amino acids

*Na+ 181mEq/L

Eusol C, Dexol, Procell are other examples

For long term storage(months)

ORGAN CULTURE

* Room temperature storage

* Upto 4 weeks

Constituents:

*Eagles MEM (Minimum essential medium)

*2% Fetal bovine serum

*Penicillin, Streptomycin, Amphotericin B

*HEPES

*Sodium bicarbonate

*L-glutamine

** European organ culture supports endothelium upto 120 days.

EUROSOL TISSUE CULTURE

Storage at 31 degree celcius for upto 1 month.

CRYOPRESERVATION

Corneas are stored at -80 degree celcius in liquid nitrpgen with dimethyl superoxide (DMSO) for indefinite period of time.

**  Grafts for lamellar keratoplasty, corneoscleral buttons are stored in 100% glycerine. Endothelium is non viable and they can be used upto 1 year.